微信小陈
微信小章| 产品名称： | Lec8 originally named Pro-5WgaRVIII3D |
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| 商品货号： | TS211643 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Tissue： | ovary |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | monolayer |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Applications： | May be useful for studying the role of galactose residues in the function and compartmentalization of glycosylated macromolecules due to a drastic reduction in the transport of UDP-galactose into the Golgi compartment. Can be usedxa0as a Pro- marker inxa0complementation studies due to Pro+ occuring at a high frequency in the population. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line is a mutant clone derived from the parental CHO clone Pro-5 (a proline auxotroph, see ATCC CRL-1781) by selection for resistance to wheat germ agglutinin. |
| Comments： | The cells exhibit a drastic reduction in the transport of UDP-galactose into the Golgi compartment. The cells are proline auxotrophs and must be grown in a medium that contains proline (40 mg/L). The population contains Pro+ revertants at high frequency (approximately 1 in 250 cells). These cells may also be grown as a suspension culture if agitated. Keep the cell concentration between 2 x 104 and 1 x 106 cells/mL. |
| Complete Growth Medium： | Alpha minimum essential medium, 90%, (GIBCO catalog #A10490-01); fetal bovine serum, 10% |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.xa0xa0 |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | P Stanley |
| Deposited As： | Cricetulus griseus |
| References： | Stanley P. Selection of specific wheat germ agglutinin-resistant (WgaR) phenotypes from Chinese hamster ovary cell populations containing numerous lecR genotypes. Mol. Cell. Biol. 1: 687-696, 1981. PubMed: 9279382 Deutscher SL, Hirschberg CB. Mechanism of galactosylation in the Golgi apparatus. A Chinese hamster ovary cell mutant deficient in translocation of UDP-galactose across Golgi vesicle membranes. J. Biol. Chem. 261: 96-100, 1986. PubMed: 3510203 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |