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| 产品名称： | LHR-1055 |
|---|---|
| 商品货号： | TS211644 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human LH/hCG receptor. |
| Derivation： | Spleen cells were fused with Sp2/0 mouse myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor, extracellular domain |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor, extracellular domain |
| Tumorigenic： | Yes |
| Effects： | Yes, forms ascites in BALB/c mice |
| Comments： | Animals were immunized with recombinant human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor expressed in E. coli. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody recognizes the extracellular domain (hormone binding domain) of the LH/hCG receptor, (amino acids domain 21-362). The LHR-29 (ATCC CRL-2685), LHR-74 (ATCC CRL-2686) and LHR-1055 (TS211644) antibodies are additive. They recognize different epitopes. The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human LH/hCG receptor. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
|
| Subculturing： | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | H Loosfelt, E Milgrom |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Meduri G, et al. Luteinizing hormone/human chorionic gonadotropin receptors in breast cancer. Cancer Res. 57: 857-864, 1997. PubMed: 9041186 |