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微信小章| 产品名称： | LBRM TG6 |
|---|---|
| 商品货号： | TS211655 |
| Organism： | Mus musculus, mouse |
| Cell Type： | T lymphocyte; radiation induce |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | B10.BR |
| Applications： | The LBRM TG6 line was derived from the LBRM 33 T cell lymphoma (see ATCC TIB-155). The cells produce IL-2 in response to IL-1 and PHA. These cells can be used to measure IL-1 activity in a very rapid and sensitive assay. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The LBRM TG6 line was derived from the LBRM 33 T cell lymphoma (see ATCC TIB-155). |
| Receptor Expression： | interleukin 1 (IL-1) |
| Genes Expressed： | interleukin 2; IL-2; interleukin-2 |
| Cellular Products： | interleukin 2; IL-2; interleukin-2 |
| Comments： | The LBRM TG6 line was derived from the LBRM 33 T cell lymphoma (see ATCC TIB-155). The cells produce IL-2 in response to IL-1 and PHA. These cells can be used to measure IL-1 activity in a very rapid and sensitive assay. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium with 0.02 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 5 cells/mL and maintain between 1 x 10 5 and 1 x 10 6 cells/mL. Medium Renewal: Two to three times weekly |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | JW Larrick |
| Deposited As： | Mus musculus |
| References： | Larrick JW, et al. An improved assay for the detection of interleukin 1. J. Immunol. Methods 79: 39-45, 1985. PubMed: 3873500 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |