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| 产品名称： | LBRM-33 clone 4A2 |
|---|---|
| 商品货号： | TS211656 |
| Organism： | Mus musculus, mouse |
| Cell Type： | T lymphocyte; radiation induce |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension; some adherent cells |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | B10.BR |
| Applications： | This line was derived from a radiation induced T cell lymphoma. Production of IL-2 is stimulated by T cell mitogens. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | This line was derived from a radiation induced T cell lymphoma. |
| Genes Expressed： | interleukin-2 (interleukin 2, IL-2) |
| Cellular Products： | interleukin-2 (interleukin 2, IL-2) |
| Comments： | This line was derived from a radiation induced T cell lymphoma. Production of IL-2 is stimulated by T cell mitogens. Peak levels are found in the culture medium after 24 hours of stimulation with either 1% PHA or 20 microgm/ml Con A. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml Adherent cells can be dislodged by scraping with subsequent resuspension as above. |
| Cryopreservation： | 90% FBS + 10% DMSO |
| Name of Depositor： | P Ralph |
| Deposited As： | Mus musculus |
| References： | Gillis S, et al. Biochemical and biologic characterization of lymphocyte regulatory molecules. III. The isolation and phenotypic characterization of interleukin-2 producing T cell lymphomas. J. Immunol. 125: 2570-2578, 1980. PubMed: 6968790 Mochizuki D, et al. Biochemical and biologic characterization of lymphocyte regulatory molecules. IV. Purification of Interleukin 2 from a murine T cell lymphoma. J. Immunol. 125: 2579-2583, 1980. PubMed: 7000903 |