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微信小章| 产品名称： | L8 |
|---|---|
| 商品货号： | TS211662 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | skeletal muscle |
| Cell Type： | myoblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Carcinogen |
| Age： | newborn |
| Applications： | transfection host |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was originally isolated by D. Yaffe in 1969 from primary rat skeletal muscle cultures. Unlike the L6 cell line (ATCC CRL-1458) no carcinogen was used to establish the L8 line. |
| Genes Expressed： | creatine phosphokinase (CPK); myosin |
| Cellular Products： | creatine phosphokinase (CPK); myosin |
| Comments： | Upon becoming confluent, L8 will fuse to form cross striated multinucleated muscle fibers. It is recommended that early passages be preserved in liquid nitrogen and that the line be recloned periodically and reselected for progeny that have the ability to fuse. It is important that the cells be subcultured when the flask is about 60% confluent. The myoblastic population will be depleted if the cultures are allowed to become confluent since most of the cells will fuse into nondividing syncytia. To avoid this, one must subculture before before the cultures become confluent and should reclone periodically and select myoblastic clones. |
| Complete Growth Medium： | A 4:1 mixture of Dulbeccos modified Eagles medium and Medium 199, 89%; chicken embryo extract (US Biological cat# C3999), 1%; horse serum, 10% |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium Renewal: Twice per week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Name of Depositor： | B Paterson |
| Deposited As： | Rattus sp. |
| Passage History： | It is recommended that early passages be preserved in liquid nitrogen and that the line be recloned periodically and reselected for progeny that have the ability to fuse. |
| References： | Richler C, Yaffe D. The in vitro cultivation and differentiation capacities of myogenic cell lines. Dev. Biol. 23: 1-22, 1970. PubMed: 5481965 Yaffe D, Saxel O. A myogenic cell line with altered serum requirements for differentiation. Differentiation 7: 159-166, 1977. PubMed: 558123 |