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微信小章| 产品名称： | LA7 |
|---|---|
| 商品货号： | TS211664 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | mammary gland |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Gender： | female |
| Strain： | Sprague-Dawley |
| Applications： | LA7 is a mammary tumor cell line derived in 1979 from a Sprague-Dawley adult female rat by Renato Dulbecco at The Salk Institute in San Diego, CA. Cells irradiated with 6000 rads may be used as feeder layers. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | LA7 is a mammary tumor cell line derived in 1979 from a Sprague-Dawley adult female rat by Renato Dulbecco at The Salk Institute in San Diego, CA. It is a clonal derivative of the Rama 25 line obtained from a rat treated with dimethylbenz(a)anthracene. |
| Comments： | LA7 is a mammary tumor cell line derived in 1979 from a Sprague-Dawley adult female rat by Renato Dulbecco at The Salk Institute in San Diego, CA. It is a clonal derivative of the Rama 25 line obtained from a rat treated with dimethylbenz(a)anthracene. Cells irradiated with 6000 rads may be used as feeder layers. Such feeder layers will stimulate the proliferation of normal primary mouse and rat mammary cells and normal primary mouse thymic epithelial cells. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with reduced sodium bicarbonate (1.5 to 2.0 g/L), 4.5 g/L glucose, 0.005 mg/ml insulin, 50 nM hydrocortisone and 20 mM HEPES, 95%; fetal bovine serum, 5%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:8 to 1:10 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | UK Ehmann |
| Deposited As： | Rattus sp. |
| Year of Origin： | 1979 |
| References： | Ehmann UK, et al. To grow mouse mammary epithelial cells in culture. J. Cell Biol. 98: 1026-1032, 1984. PubMed: 6699079 Dulbecco R, et al. Differentiation of a rat mammary cell line in vitro. Proc. Natl. Acad. Sci. USA 76: 1256-1260, 1979. PubMed: 375233 Ehmann UK, et al. Long-term proliferation of mouse thymic epithelial cells in culture. In Vitro Cell. Dev. Biol. 22: 738-748, 1986. PubMed: 3782011 Ehmann UK, et al. Cultured proliferating rat mammary epithelial cells. In Vitro Cell. Dev. Biol. 27A: 749-754, 1991. PubMed: 1717432 Ehmann UK, et al. Gap-junctional communication between feeder cells and recipient normal epithelial cells correlates with growth stimulation. In Vitro Cell. Dev. Biol. Anim. 37: 100-110, 2001. PubMed: 11332735 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |