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微信小章| 产品名称： | LADMAC |
|---|---|
| 商品货号： | TS211665 |
| Organism： | Mus musculus, mouse |
| Tissue： | bone marrow |
| Cell Type： | macrophage, monocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension, with some loosely adherent cells |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Strain： | C3H |
| Applications： | This cell line is used to produce LADMAC conditioned medium. It will support the growth of the macrophage cell lines EOC 2 (ATCC CRL-2467), EOC 13.31 (ATCCxa0CRL-2468), EOC 20 (ATCC CRL-2469), I-11.15 (ATCC CRL-2470) and I-13.35 (ATCCxa0CRL-2471). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | LADMAC is a transformed cell line derived by transfecting mouse bone marrow cells highly enriched for macrophage progenitors with cloned human cellular myc-homologous sequences covalently attached to pBR325 (pR myc). |
| Genes Expressed： | colony stimulating factor-1 (CSF-1) |
| Cellular Products： | colony stimulating factor-1 (CSF-1) |
| Tumorigenic： | Yes |
| Effects： | Yes, the cells are tumorigenic in nu+, nu+ mice but not in syngenic mice. |
| Comments： | The cell line has monocyte-like morphology; contains nonspecific esterase; is phagocytic for latex beads; secretes lysozyme, and bears the Mac-1 antigen.
A minority of cells are Fc receptor positive and an appreciable number of cells are complement receptor 1 positive. The cells are tumorigenic in nu+, nu+ mice but not in syngenic mice. The cells are not phagocytic for antibody or complement-coated particles; they do not constitutively secrete Interleukin-1. LADMAC cells secrete the growth factor colony stimulating factor 1 (CSF-1). CSF-1 is capable of supporting the in vitro proliferation of mouse bone marrow macrophages. The Pannell-Milstein roller bottle apparatus may be used to produce high concentrations of CSF-1. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. Attached cells may be subcultured by tapping the sides of the flask until cells are dispersed. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).xa0 Generation of conditioned Medium
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| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Name of Depositor： | WS Walker |
| Deposited As： | mouse |
| References： | Sklar MD, et al. Transformation of mouse bone marrow cells by transfection with a human oncogene related to c-myc is associated with the endogenous production of macrophage colony stimulating factor 1. J. Cell. Physiol. 125: 403-412, 1985. PubMed: 3877730 Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247 |
Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247