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| 产品名称： | L368 |
|---|---|
| 商品货号： | TS211668 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human beta-2-microglobulin (beta 2 microglobulin) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human beta-2-microglobulin (beta 2 microglobulin) |
| Comments： | Animals were immunized with the RPMI 8866 human B cell line. Spleen cells were fused with NS-1 myeloma cells. The antibody does not cross-react with mouse, rat, cat or monkey beta-2-microglobulin. The antibody is not cytotoxic. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 3 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | LA Lampson |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Lampson LA, et al. Striking paucity of HLA-A, B, C and beta2-microglobulin on human neuroblastoma cell lines. J. Immunol. 130: 2471-2478, 1983. PubMed: 6187860 |