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| 产品名称： | L5.1 |
|---|---|
| 商品货号： | TS211672 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | leukemia |
| Strain： | Strain: BALB/c (B cell); BALB/c (myeloma) |
| Applications： | The antibody reacts strongly with all morphologically recognizable precursors of the human erythroid lineage. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human transferrin receptor |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human transferrin receptor |
| Comments： | Animals were immunized with the HL-60 promyelocytic leukemia cell line. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody precipitates an 87000 dalton glycoprotein present on the surface of K562 leukemia cells. The antibody reacts strongly with all morphologically recognizable precursors of the human erythroid lineage. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG2a |
| Name of Depositor： | G Rovera |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Lebman D, et al. A monoclonal antibody that detects expression of transferrin receptor in human erythroid precursor cells. Blood 59: 671-678, 1982. PubMed: 7059675 |