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| 产品名称： | L227 |
|---|---|
| 商品货号： | TS211674 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with a non-polymorphic Ia like antigen on human cells. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human Ia |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human Ia |
| Comments： | Animals were immunized with RPMI 8866 cells (a B lymphoblastoid cell line). Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with a non-polymorphic Ia like antigen on human cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Complete culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Isotype： | IgG1 kappa , IgG1; kappa light chain |
| Name of Depositor： | LA Lampson |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Lampson LA, Levy R. Two populations of Ia-like molecules on a human B cell line. J. Immunol. 125: 293-299, 1980. PubMed: 6966655 |