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微信小章| 产品名称： | L5178-R (LY-R) |
|---|---|
| 商品货号： | TS211675 |
| Organism： | Mus musculus, mouse |
| Tissue： | thymus |
| Cell Type： | chemically induced |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | DBA/2 |
| Applications： | The cells are resistant to X-rays, but sensitive to ultraviolet radiation, heat and some potential anti neoplastic drugs (e.
The X-ray sensitive L5178-S line (ATCC CRL-1723) was derived from this line.
Tested and found negative for ectromelia virus. |
| Derivation： | This cell line was derived from the L5178 thymic lymphoma induced by methylcholanthrene in a DBA/2 mouse. The X-ray sensitive L5178-S line (ATCC CRL-1723) was derived from this line. |
| Tumorigenic： | Yes |
| Effects： | Yes, in DBA/2 mice |
| Comments： | The cells are resistant to X-rays, but sensitive to ultraviolet radiation, heat and some potential anti neoplastic drugs (e.g., cis-PAD). After 6 months to 3 years in culture, the line converts to an X-ray sensitive state. Tested and found negative for ectromelia virus. |
| Complete Growth Medium： | Fischers medium with 0.1 g/L sodium pyruvate and 1.125 g/L sodium bicarbonate, 90%; horse serum, 10%
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 x 10 4 cells/mL and maintain between 3 x 10 4 and 7 x 10 4 cells/mL.
Medium Renewal: Twice per week |
| Cryopreservation： | culture medium, 90%; DMSO, 10% |
| Name of Depositor： | JZ Beer |
| Deposited As： | Mus musculus |
| References： | Beer JZ, et al. X-ray and UV mutagenesis in two L5178Y cell strains differing in tumorigenicity, radiosensitivity, and DNA repair. Br. J. Cancer Suppl. 6: 107-111, 1984. PubMed: 6582899 Beer JZ, et al. Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells. Cancer Res. 43: 4736-4742, 1983. PubMed: 6883332 Beer JZ, et al. Effects of low dose rate (0.003-0.025 Gy/h) chronic X-irradiation on radioresistant and radiosensitive L5178Y mouse lymphoma cells. Int. J. Radiat. Biol. 48: 609-619, 1985. PubMed: 3876312 |