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| 产品名称： | L5178Y TK+/- clone (3.7.2C) TK+/- (clone 3.7.2C) |
|---|---|
| 商品货号： | TS211677 |
| Organism： | Mus musculus, mouse |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | DBA/2 |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | This line is a derivative of L5178Y-S (ATCC CRL-1723). |
| Comments： | This line is a derivative of L5178Y-S (ATCC CRL-1723). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.1% Pluronic, 90%; fetal bovine serum, 10%
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 to 2 x 105 viable cells/mL.
Maintain cell density between 2 x 105 and 1 x 106 viable cells/mL.
Medium Renewal: Every 3 to 4 days |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | Mobil Oil Corporation |
| Deposited As： | Mus musculus |
| U.S. Patent Number： | |
| References： | . . Chem. Mutagens 3: pp. 79-103, 1973. |