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| 产品名称： | L11/135 |
|---|---|
| 商品货号： | TS211679 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with an antigen present on all rabbit T cells. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with P3X63Ag8-U1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against rabbit T lymphocytes (T cells) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against rabbit T lymphocytes (T cells) |
| Comments： | Animals were immunized with cell surface glycoproteins of the rabbit T cell line, RL-5. Spleen cells were fused with P3X63Ag8-U1 myeloma cells. The antibody reacts with an antigen present on all rabbit T cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | S Jackson |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Jackson S, et al. Differentiation antigens identify subpopulations of rabbit T and B lymphocytes. J. Exp. Med. 157: 34-46, 1983. PubMed: 6600269 |