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微信小章| 产品名称： | L1210 |
|---|---|
| 商品货号： | TS211680 |
| Organism： | Mus musculus, mouse |
| Tissue： | skin |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphocytic leukemia |
| Age： | 8 months |
| Gender： | female |
| Strain： | DBA subline 212 |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 40; range = 32 to 80. Mouse karyotype with a diploid stemline number of 40 chromosomes. |
| Derivation： | The line was established from a tumor in female #234 that developed following skin paintings with 0.2% methylcholanthrene in ether. |
| Clinical Data： | female
|
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice
|
| Comments： | Tested and found negative for ectromelia virus (mousepox).
|
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 x 10xa04 viable cells/mL.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 4 days |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Population Doubling Time： | 8 to 10 hrs |
| Name of Depositor： | EM Jensen |
| Deposited As： | Mus musculus |
| References： | Law LW, et al. Observations on the effect of a folic-acid antagonist on transplantable lymphoid leukemias in mice. J. Natl. Cancer Inst. 10: 179-192, 1949. PubMed: 18143260 Moore GE, et al. Suspension Cell Culture and In Vivo and In Vitro Chromosome Constitution of Mouse Leukemia L1210. J. Natl. Cancer Inst. 36: 405-421, 1966. PubMed: 18630317 Himmelfarb P, et al. Growth of Colonies of. Murine Leukemia L1210 in Vitro. Cancer Chemother. Rep. 51: 451-3 1967 Young SW, et al. Gadolinium(III) texaphyrin: a tumor selective radiation sensitizer that is detectable by MRI. Proc. Natl. Acad. Sci. USA 93: 6610-6615, 1996. PubMed: 8692865 |