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微信小章| 产品名称： | L-132 |
|---|---|
| 商品货号： | TS211682 |
| Organism： | Homo sapiens, human |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain Human papillomavirus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | This line was originally thought to be derived from embryonic lung tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. The cells are positive for keratin by immunoperoxidase staining. |
| Derivation： | This line was originally thought to be derived from embryonic lung tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. |
| HeLa Markers： | Y |
| Genes Expressed： | keratin,The cells are positive for keratin by immunoperoxidase staining. |
| Cellular Products： | keratin |
| Comments： | This line was originally thought to be derived from embryonic lung tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. The cells are positive for keratin by immunoperoxidase staining. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended Medium Renewal: Twice per week Remove medium, add fresh 0.25% trypsin solution for 1 to 3 minutes. Remove trypsin and let culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Culture Conditions： | Temperature: 37.0°C |
| STR Profile： | Amelogenin: X CSF1PO: 9,10 D13S317: 12,13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
| Isoenzymes： | G6PD, A |
| Name of Depositor： | EV Davis |
| Deposited As： | Homo sapiens |
| References： | Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708 Erfle V, Mellert W. Human cell line LC5 and its use. US Patent 5,582,967 dated Dec 10 1996 . . Fed. Proc. 19: 386, 1960. . . Science 133: 2059, 1961. Tresnan DB, et al. Feline aminopeptidase N serves as a receptor for feline, canine, porcine, and human coronaviruses in serogroup I. J. Virol. 70: 8669-8674, 1996. PubMed: 8970993 |