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微信小章| 产品名称： | KU812F KU-812-F |
|---|---|
| 商品货号： | TS211683 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | basophil |
| Product Format： | frozen |
| Morphology： | myeloblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | chronic myelogenous leukemia |
| Age： | 38 years adult |
| Gender： | male |
| Ethnicity： | Japanese |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | 58, XY, t(9;22)(q34;q11), i(17q), +1, +4, +6, +6, +8, +der(9)t(9;22), + der(9)t(9;22), +der(11)q(11), +14, +15, +19, +21, +21, -9 PubMed: 85212346 |
| Derivation： | KU812F is a clone of KU812 (ATCC CRL-2099). The parent KU812 cell line was established from the peripheral blood of a patient in blast crisis of chronic myelogenous leukemia. |
| Genes Expressed： | Transforming growth factor beta (TGF-beta) Ref |
| Cellular Products： | Transforming growth factor beta (TGF-beta) PubMed: 2698048 |
| Comments： | The cells contain at least one Ph1 (Philadelphia) chromosome. The cell line has some characteristics of basophilic leukocytes (Fc receptors, basophilic granules, histamine production), and are negative for lymphoid markers.xa0Ref |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at a cell concentration between 3 x 105 and 3 x 106 cells/mL. Do not allow the cell concentration to exceed 3 x 106 cells/mL.
Medium Renewal: Add fresh medium every two to three days (depending on cell density). |
| Cryopreservation： | Complete growth medium supplemented with 5% (v/v). xa0 |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | K Kishi |
| References： | Kishi K. A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors. Leuk. Res. 9: 381-390, 1985. PubMed: 3858609 Okano H, et al. Hemoglobin synthesis of both adult and fetal types in a human CML cell line. J. Biochem. 104: 162-164, 1988. PubMed: 2460439 Okamura S, et al. Expression of cytokine genes in hematological malignancies. Nippon Ketsueki Gakkai Zasshi 52: 1423-1432, 1989. PubMed: 2698048 KU812F cells are capable of continuous erythroid differentiation as evidenced by producion of glycophorin A, carbonic anhydrase and adult and fetal types of hemoglobin. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
Okamura S, et al. Expression of cytokine genes in hematological malignancies. Nippon Ketsueki Gakkai Zasshi 52: 1423-1432, 1989. PubMed: 2698048