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微信小章| 产品名称： | L2 |
|---|---|
| 商品货号： | TS211687 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | lung |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Gender： | female |
| Strain： | LEW/Crl |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 77; range = 49 to 85. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Clinical Data： | female |
| Comments： | Gamma glutamylcysteine synthetase activity is stimulated by oxidative stress.
According to the depositor’s reference the cells are derived from adult female Lewis strain of rats.
Ref  Douglas WH, Kaighn ME. Clonal isolation of differentiated rat lung cells. In Vitro 10: 230-237, 1974. PubMed: 4616002 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | ME Kaighn |
| Deposited As： | Rattus sp. |
| References： | Yang J, et al. Identification of an 11-residue portion of CTP-phosphocholine cytidylyltransferase that is required for enzyme-membrane interactions. Biochem. J. 325: 29-38, 1997. PubMed: 9224626 Su WY, et al. Lung epithelial cell-released nitric oxide protects against PMN-mediated cell injury. Am. J. Physiol. 271: L581-L586, 1996. PubMed: 8897905 Pei L. Identification of a negative glucocorticoid response element in the rat type 1 vasoactive intestinal polypeptide receptor gene. J. Biol. Chem. 271: 20879-20884, 1996. PubMed: 8702844 Shi MM, et al. Quinone-induced oxidative stress elevates glutathione and induces gamma- glutamylcysteine synthetase activity in rat lung epithelial L2 cells. J. Biol. Chem. 269: 26512-26517, 1994. PubMed: 7929374 Douglas WH, Kaighn ME. Clonal isolation of differentiated rat lung cells. In Vitro 10: 230-237, 1974. PubMed: 4616002 |