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| 产品名称： | KMC8.8 |
|---|---|
| 商品货号： | TS211692 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with mouse CD9 (expressed by bone marrow stromal cells, megakaryocytes, platelets, myeloid cells and subpopulations of mature lymphocytes. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse CD9 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse CD9 |
| Tumorigenic： | Yes |
| Effects： | Yes, produces only modest amounts of ascites in SCID mice because it usually fatal within two weeks of inoculation |
| Comments： | Animals were immunized with BMS2 murine bone marrow stromal cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with mouse CD9 (expressed by bone marrow stromal cells, megakaryocytes, platelets, myeloid cells and subpopulations of mature lymphocytes. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgG2a |
| Name of Depositor： | PW Kincade |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Oritani K, et al. Antibody ligation of CD9 modifies production of myeloid cells in long-term cultures. Blood 87: 2252-2261, 1996. PubMed: 8630385 |