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微信小章| 产品名称： | KG-1a |
|---|---|
| 商品货号： | TS211700 |
| Organism： | Homo sapiens, human |
| Tissue： | bone marrow |
| Cell Type： | promyeloblast, Macrophage |
| Product Format： | frozen |
| Morphology： | myeloblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | acute myelogenous leukemia |
| Age： | 59 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | The stemline chromosome number is 46 (pseudodiploid), with the 2S component occurring at 5.8%. Seven markers, including five ATCC CCL-246-specific markers, were found in most, if not all metaphases analyzed., Another marker ? del (7) was found only in about 50% of the metaphases. Normal chromosomes 5, 7, 8, 12, 17 and 22 were monosomic. The Y chromosome is detected in the Q-banded preparations. |
| Derivation： | The variant subline KG-1a of the human acute myelogenous leukemia cell line KG-1 was isolated by H.P. Koeffler, et al. |
| Clinical Data： | 59 years Caucasian male |
| Comments： | After the tenth passage, the parental KG-1 cells were cultured in two separate laboratories within the same department under identical conditions. After 35 passages the cells in one laboratory expressed morphological differences from the parent line. The variant KG-1a was composed of undifferentiated promyeloblasts. The cells did not stain for ASD chloroacetate esterase, alpha-naphthyl butyrate esterase or peroxidase.
Both populations exhibit many common characteristics. They share a similar doubling time, are negative for EBNA and VCA, express no surface immunoglobulins and exhibit identical HLA and isoenzyme profiles. In contrast to the parental KG-1 (ATCC CCL-246) the KG-1a population is unresponsive to colony-stimulating factor in soft-agar culture and does not express the Ia-like antigen. KG-1a cells are resistant to phorbol diester induced macrophage differentiation and proliferation of the cells is unaffected by the presence of phorbol diesters .The KG-1a cells are morphologically, cytochemically, and functionally less mature than the parental KG-1. Although derived from, and almost identical to, KG-1 (ATCC CCL-246) these cells do not spontaneously differentiate to granulocyte and macrophage like cells, do not express DR and do not respond to colony stimulating factor (CSF). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL.
Maintain cell density between 2 x 105 and 1 x 106 viable cells/mL. Medium Renewal:xa0Twice per week |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 7 D13S317: 11,12 D16S539: 10,11 D5S818: 13 D7S820: 8,10 THO1: 7,8 TPOX: 7,9 vWA: 14,19 |
| Isoenzymes： | AK-1, 0 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1-2 PGM3, 0 |
| Name of Depositor： | DW Golde |
| Deposited As： | Homo sapiens |
| Passage History： | After the tenth passage, the parental KG-1 cells were cultured in two separate laboratories within the same department under identical conditions. After 35 passages the cells in one laboratory expressed morphological differences from the parent line. The variant KG-1a was composed of undifferentiated promyeloblasts. |
| References： | Koeffler HP. Induction of differentiation of human acute myelogenous leukemia cells: therapeutic implications. Blood 62: 709-721, 1983. PubMed: 6192859 Koeffler HP, et al. An undifferentiated variant derived from the human acute myelogenous leukemia cell line (KG-1). Blood 56: 265-273, 1980. PubMed: 6967340 Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679 Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022 |