微信小陈
微信小章| 产品名称： | KB |
|---|---|
| 商品货号： | TS211710 |
| Organism： | Homo sapiens, human |
| Tissue： | HeLa contaminant |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain human papilloma virus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Carcinoma, Papilloma |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was originally thought to be derived from an epidermal carcinoma of the mouth, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. |
| HeLa Markers： | Y |
| Genes Expressed： | keratin |
| Comments： | The cells are positive for keratin by immunoperoxidase staining.
KB cells have been reported to contain human papillomavirus 18 (HPV-18) sequences.
NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 9,10 D13S317: 12,13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
| Isoenzymes： | G6PD, A |
| Name of Depositor： | H Eagle |
| Deposited As： | Homo sapiens |
| References： | Eagle H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc. Soc. Exp. Biol. Med. 89: 362-364, 1955. PubMed: 13254761 Boshart M, et al. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J. 3: 1151-1157, 1984. PubMed: 6329740 Salzman NP. Animal Cell Cultures. Science 133:1559-65. 1961 PubMed: 13745868 Eagle H, Foley GE. Cytotoxicity in human cell cultures as a primary screen for the detection of anti-tumor agents. Cancer Res. 18: 1017-1025, 1958. PubMed: 13596943 Foley GE, Handler AH. Differentiation of “Normal” and Neoplastic Cells Maintained in Tissue Culture by Implantation into Normal Hamsters. Proc Soc Exp Biol Med 94: 661-664 1957. PubMed: 13431913 Eagle H, et al. Viral susceptibility of a human carcinoma cell (strain KB). Proc. Soc. Exp. Biol. Med. 91: 361-364, 1956. PubMed: 13322936 St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830 Chang K, Pastan I. Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140, 1996. PubMed: 8552591 Lee RJ, Huang L. Folate-targeted, anionic liposome-entrapped polylysine-condensed DNA for tumor cell-specific gene transfer. J. Biol. Chem. 271: 8481-8487, 1996. PubMed: 8626549 |
| Cross References： | Nucleotide (GenBank) : AW062989 EST00001 PT-PCR products Homo sapiens cDNA clone K-5, mRNA sequence. Nucleotide (GenBank) : AW062990 EST00002 PT-PCR products Homo sapiens cDNA clone K-13, mRNA sequence. Nucleotide (GenBank) : AW062991 EST00003 PT-PCR products Homo sapiens cDNA clone K-16, mRNA sequence. Nucleotide (GenBank) : AW062992 EST00004 PT-PCR products Homo sapiens cDNA clone K-32, mRNA sequence. Nucleotide (GenBank) : AW062993 EST00005 PT-PCR products Homo sapiens cDNA clone K-33, mRNA sequence. Nucleotide (GenBank) : AW062994 EST00006 PT-PCR products Homo sapiens cDNA clone K-34, mRNA sequence. Nucleotide (GenBank) : AW062995 EST00007 PT-PCR products Homo sapiens cDNA clone K-45, mRNA sequence. |