微信小陈
微信小章| 产品名称： | K-BALB (K-234) |
|---|---|
| 商品货号： | TS211712 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Cell Type： | fibroblast, Kirsten murine sarcoma virus (Ki-MSV) transformed |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 14 to 17 days gestation embryo |
| Strain： | BALB/c |
| Storage Conditions： | liquid nitrogen vapor phase |
| Antigen Expression： | H-2d |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | They do not produce Ki-MSV. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium, 90%; bovine calf serum, 10%
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:8 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete culture medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | S Aaronson |
| Deposited As： | Mus musculus |
| References： | Aaronson SA, Weaver CA. Characterization of murine sarcoma virus (Kirsten) transformation of mouse and human cells. J. Gen. Virol. 13: 245-252, 1971. PubMed: 4333712 Stephenson JR, Aaronson SA. Antigenic properties of murine sarcoma virus-transformed BALB-3T3 nonproducer cells. J. Exp. Med. 135: 503-515, 1972. PubMed: 4550769 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |