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微信小章| 产品名称： | KATO III |
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| 商品货号： | TS211714 |
| Organism： | Homo sapiens, human |
| Tissue： | stomach; derived from metastatic site: pleural effusion and supraclavicular and axillary lymph nodes and Douglas cul-de-sac |
| Product Format： | frozen |
| Morphology： | spherical |
| Culture Properties： | mixed, adherent and suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | gastric carcinoma |
| Age： | 55 years adult |
| Gender： | male |
| Ethnicity： | Asian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | The stemline chromosome number is hypotetraploid with the 2S component occurring at 6.2%. Nine markers were common to most S metaphases, four markers were less frequent. One (occasionally 2 copies) homogenous staining region (HSR) (t(11;HSR) was present in all metaphases examined, but no double minutes (DM) were detected. |
| Images： |  |
| Derivation： | A human gastric carcinoma cell line, KATO-III, was established in vitro from a pleural effusion of a 55-year-old male patient. |
| Clinical Data： | 55 years adult Asian male |
| Tumorigenic： | Yes |
| Effects： | Yes, in cheek pouches of anti thymocyte serum treated hamsters No, in nude mice |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: 5% CO2 in air recommended Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 7,11 D13S317: 8,12 D16S539: 10,12 D5S818: 10,11 D7S820: 8,12 THO1: 7,9 TPOX: 11 vWA: 14,16 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 PGM1, 1 PGM3, 1 |
| Name of Depositor： | M Sekiguchi |
| Deposited As： | Homo sapiens |
| References： | Sekiguchi M, et al. Establishment of cultured cell lines derived from a human gastric carcinoma. Jpn. J. Exp. Med. 48: 61-68, 1978. PubMed: 209229 Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216 Rieder G, et al. Role of adherence in Interleukin-8 induction in Helicobacter pylori-associated gastritis. Infect. Immun. 65: 3622-3630, 1997. PubMed: 9284128 Mycoplasma contamination was detected in cultures submitted to the ATCC and was eliminated in 1983. |