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微信小章| 产品名称： | KEL FIB |
|---|---|
| 商品货号： | TS211716 |
| Organism： | Homo sapiens, human |
| Tissue： | skin/connective tissue |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | keloid |
| Age： | 35 years |
| Gender： | female |
| Ethnicity： | Black |
| Storage Conditions： | liquid notrogen vapor phase |
| Clinical Data： | 35 years Black female |
| Genes Expressed： | collagen |
| Cellular Products： | collagen |
| Comments： | Progeny from the current distribution stock (passage 11) have been shown to undergo at least 10 population doublings from the frozen state. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommendedxa0 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| STR Profile： | Amelogenin: X CSF1PO: 8,12 D13S317: 11,13 D16S539: 9 D5S818: 12 D7S820: 8,10 THO1: 7,8 TPOX: 8,11 vWA: 14,16 |
| Name of Depositor： | M Babu, RF Diegelmann, I Cohen |
| Deposited As： | Homo sapiens |
| Passage History： | Progeny from the current distribution stock (passage 11) have been shown to undergo at least 10 population doublings from the frozen state. |
| References： | Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |