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微信小章| 产品名称： | JEG-3 |
|---|---|
| 商品货号： | TS211718 |
| Organism： | Homo sapiens, human |
| Tissue： | placenta |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | choriocarcinoma |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a hypertriploid human cell line. The modal chromosome number is 71, occurring at 34%, and polyploidy at 2.6%. The t(4;11)(p15;q13), i(13q), t(10p15q), del(18)(q21), and 6 other markers are common to most cells, and two other markers are found in some. Giant satellites are seen in one N14, and two N22. N2, N5, and N9 have 4 copies, and N7, N13, N18, N21 and X a single copy. A single Y chromosome is detected by Q-band examination. |
| Images： |  |
| Derivation： | This is one of six clonally derived lines isolated from the Woods strain of the Erwin-Turner tumor by Kohler and associates. |
| Genes Expressed： | human chorionic gonadotropin (hCG), human chorionic somatomammotropin (placental lactogen); progesterone |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms malignant tumor consistent with choriocarcinoma |
| Comments： | The cells are able to transform steroid precursors to estrone and estradiol. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 9,11 D16S539: 13,14 D5S818: 10,11 D7S820: 10,12 THO1: 9,9.3 TPOX: 8 vWA: 16 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 PGM1, 1 PGM3, 1-2 |
| Name of Depositor： | G Kohler |
| Deposited As： | Homo sapiens |
| References： | Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 . . Acta Endocrinol. Suppl. 153: 137-153, 1971. Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029 Roesler WJ, et al. The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the phosphoenolpyruvate carboxykinase promoter in hepatoma cells. J. Biol. Chem. 271: 8068-8074, 1996. PubMed: 8626491 Kohler PO, Bridson WE. Isolation of hormone-producing clonal lines of human choriocarcinoma. J. Clin. Endocrinol. 32: 683-687, 1971. |