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微信小章| 产品名称： | JM1 |
|---|---|
| 商品货号： | TS211719 |
| Organism： | Homo sapiens, human |
| Tissue： | blood |
| Cell Type： | pre-B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | B cell lymphoma |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | The JM1 cell line was established from cells taken from a patient with immunoblastic B cell lymphoma - leukemia. |
| Antigen Expression： | HLA-DR; Homo sapiens, expressed CD19; Homo sapiens, expressed CD10; Homo sapiens, expressed |
| Genes Expressed： | CD19; Homo sapiens, expressed ,CD10; Homo sapiens, expressed ,HLA-DR; Homo sapiens, expressed |
| Comments： | The cells are negative for CD7, My 7, My 9 and surface IgM. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Alternatively, cultures may be established by centrifugation. Adherent cells can be dislodged by scraping. Initiate cultures at 2 to 5 x 105 viable cells/mL. Subculture when cell density reaches 1 x 106 cells/mL.
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Complete culture medium, 95%; DMSO 5%. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| STR Profile： | D5S818: 12 D13S317: 11 D7S820: 8, 10 D16S539: 9 vWA: 17 THO1: 6 TPOX: 10, 11 CSF1PO: 12 Amelogenin: X Y |
| Name of Depositor： | Immunex Corporation |
| Deposited As： | Homo sapiens |
| U.S. Patent Number： | |
| References： | Park LS, et al. Heterogeneity in human interleukin-3 receptors. A subclass that binds human granulocyte/macrophage colony stimulating factor. J. Biol. Chem. 264: 54205427, 1989. PubMed: 2647717 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |