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微信小章| 产品名称： | Jensen Sarcoma |
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| 商品货号： | TS211721 |
| Organism： | Rattus norvegicus, rat |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Virus Resistance： | poliovirus 2 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended Medium Renewal: Every day (when possible) Place culture in cold (+4C) for 60 minutes, shake flask to suspend cells, centrifuge, resuspend in fresh medium and dispense into new flasks. |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Name of Depositor： | MK Patterson |
| Deposited As： | Rattus sp. |
| References： | Proc. Soc. Exp. Biol. Med. 100: 115, 1959. . . Z. Krebsforsch 7: 45, 1908. Patterson MK Jr., Orr G. L-asparagine biosynthesis by nutritional variants of the Jensen sarcoma. Biochem. Biophys. Res. Commun. 26: 228-233, 1967. PubMed: 6067662 Patterson MK Jr., et al. Studies on the asparagine requirement of the Jensen sarcoma and the derivation of its nutritional variant. Cancer Res. 29: 296-300, 1969. PubMed: 5765412 Patterson MK Jr., et al. Studies on the aspartic acid "sparing effect" on the nutritional requirement of L-asparagine for tumors in vitro. Proc. Soc. Exp. Biol. Med. 131: 131-134, 1969. PubMed: 5770086 |