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微信小章| 产品名称： | Jiyoye Jijoye, P-2003, P-3J |
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| 商品货号： | TS211722 |
| Organism： | Homo sapiens, human |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells Contain HERPESVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Burkitts lymphoma |
| Age： | 7 years |
| Gender： | male |
| Ethnicity： | Black, African |
| Storage Conditions： | liquid nitrogen vapor phase |
| Antigen Expression： | HLA A32, B17, Bw37 |
| Genes Expressed： | Epstein-Barr virus (EBV) |
| Cellular Products： | Epstein-Barr virus (EBV) |
| Virus Resistance： | The cells are partially resistant to poliovirus and vesicular stomatitis viruses. |
| Comments： | EBNA positive. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 4 to 6 x 105 viable cells/mL. A maximum of 2 - 3 x 106 viable cells/mL is obtainable.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 12 D16S539: 10,11 D5S818: 12 D7S820: 8,10 THO1: 7,9 TPOX: 6,8 vWA: 15,19 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | W Henle |
| Deposited As： | Homo sapiens |
| References： | . . J. Clin. Pathol. 38: 261, 1985. Kohn G, et al. Involvement of C group chromosomes in five Burkitt lymphoma cell lines. J. Natl. Cancer Inst. 38: 209-222, 1967. PubMed: 6019732 . . In Vitro 4: 165, 1969. Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |