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微信小章| 产品名称： | Jurkat, Clone E6-1 |
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| 商品货号： | TS211725 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | acute T cell leukemia |
| Gender： | male |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a pseudodiploid human cell line. The modal chromosome number is 46, occurring in 74% with polyploidy at 5.3%. The karyotype is 46,XY,-2,-18,del(2) (p21p23),del(18) (p11.2). Most cells had normal X and Y chromosomes. |
| Images： |  |
| Derivation： | The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM. The line was cloned from cells obtained from Dr. Kendall Smith and are mycoplasma free. This is a clone of the Jurkat-FHCRC cell line, a derivative of the Jurkat cell line. |
| Clinical Data： | male The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al. |
| Antigen Expression： | CD3; Homo sapiens, expressed |
| Receptor Expression： | Receptor expression: T cell antigen receptor, expressed |
| Genes Expressed： | interleukin-2 (interleukin 2, IL-2),CD3; Homo sapiens, expressed |
| Comments： | Clone E6-1 cells produce large amounts of IL-2 after stimulation with phorbol esters and either lectins or monoclonal antibodies against the T3 antigen (both types of stimulants are needed to induce IL-2 production. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Do not allow the cell density to exceed 3 X 106 cells/mL.xa0Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Interval: Maintain cultures at a cell concentraion between between 1 x 105 and 1 X 106 viable cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,12 D16S539: 11 D5S818: 9 D7S820: 8,12 THO1: 6,9.3 TPOX: 8,10 vWA: 18 |
| Population Doubling Time： | 48 hrs |
| Name of Depositor： | A Weiss |
| Deposited As： | Homo sapiens |
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