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微信小章| 产品名称： | JAA-F11 |
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| 商品货号： | TS211728 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Adenocarcinoma |
| Applications： | The hybridoma cell line JAA-F11 was established in 1995. The hybridoma produces an antibody (IgG3) that binds the saccharide antigen, Gal beta1-3GalNAc (T-antigen). The antibody binds to breast adenocarcinoma tissue but not to normal tissues. It may be useful in tumor studies, germinal center studies and T cell maturation studies. The JAA-F11 cell line was formed by the fusion of P3X63Ag8.653 mouse myeloma cells with splenocytes from a BALB/c mouse inoculated with a synthetic Gal beta1-3GalNAc-BSA conjugate. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The hybridoma cell line JAA-F11 was established in 1995. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the saccharide antigen, Gal beta1-3GalNAc (T antigen) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the saccharide antigen, Gal beta1-3GalNAc (T antigen) |
| Comments： | The hybridoma cell line JAA-F11 was established in 1995. The hybridoma produces an antibody (IgG3) that binds the saccharide antigen, Gal beta1-3GalNAc (T-antigen). The saccharide antigen, Gal beta1-3GalNAc is of biologic importance in many systems. It is a tumor-associated carbohydrate antigen, a temporally expressed antigen in germinal center B cells and cortical T cells, a parasite-associated antigen, a spermatozoa vitality marker and an antigen on aged red blood cells. The antibody binds to breast adenocarcinoma tissue but not to normal tissues. It may be useful in tumor studies, germinal center studies and T cell maturation studies. The JAA-F11 cell line was formed by the fusion of P3X63Ag8.653 mouse myeloma cells with splenocytes from a BALB/c mouse inoculated with a synthetic Gal beta1-3GalNAc-BSA conjugate. |
| Complete Growth Medium： | Dulbeccos modified Eagles Medium with 4 mM L-glutamine adjusted to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonate and supplemented with: 0.1 mM NEAA, 1.0% MEM Vitamins and 10% fetal bovine serum
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2-3 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 2 x 105 and 5 x 105 cells/mL.xa0 Do not allow the cell concentration to exceed 5 x 105 cells/mL. Medium Renewal:xa0Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG3; kappa light chain |
| Name of Depositor： | K Rittenhouse-Diakun |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| Year of Origin： | 1995 |
| References： | Rittenhouse-Diakun K, et al. Development and characterization of monoclonal antibody to T-antigen: (gal beta1-3GalNAc-alpha-O). Hybridoma 17: 165-173, 1998. PubMed: 9627057 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |