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| 产品名称： | J82 |
|---|---|
| 商品货号： | TS211730 |
| Organism： | Homo sapiens, human |
| Tissue： | urinary bladder |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | transitional cell carcinoma |
| Age： | 58 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | The cell line is aneuploid human male (XY), with most chromosome counts in the triploid range. However, the chromosome count range is quite broad, extending from hyperdiploid to hexaploid. Normal chromosomes N11 and N20 are under-represented with respect to the other normal chromosomes: altered forms of these two chromosomes are prominent as marker chromosomes. Chromosome N13 tends towards over-representation. Five marker chromosomes are noted: 20q+, 11q+, 8p+, del(1)(q31), 5p+(HSR). One of these, 20q, is identical to marker chromosome M1 of C. OToole, et al., Br. J. Cancer 38: 64, 1978. The remainder are not as clearly related to the original marker chromosomes noted by those authors. |
| Clinical Data： | male 58 years Caucasian, Swedish |
| Antigen Expression： | HLA A2, Aw32, B5, B12, Cw5; Blood Type A |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice |
| Comments： | Electron microscopic examination did not reveal desmosomes but varying amounts of rough endoplasmic reticulum and prominent microfilaments were observed. Contains the ras (H-ras) oncogene. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 10,12 D16S539: 11,12 D5S818: 12,13 D7S820: 9,11 THO1: 9.3 TPOX: 11,12 vWA: 17,18 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1-2 PGM1, 1 PGM3, 2 |