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微信小章| 产品名称： | J774A.1 |
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| 商品货号： | TS211734 |
| Organism： | Mus musculus, mouse |
| Tissue： | ascites |
| Cell Type： | monocyte; macrophage |
| Product Format： | frozen |
| Morphology： | macrophage |
| Culture Properties： | mostly adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | reticulum cell sarcoma |
| Age： | adult |
| Gender： | female |
| Strain： | BALB/cN |
| Applications： | Biological response transfection host |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Clinical Data： | female |
| Receptor Expression： | Complement (C3), expressedxa0Ref   Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031,xa0Fc receptor, IgG, high affinity I (Fcgr1), expressedxa0RefSears DW, et al. Molecular cloning and expression of the mouse high affinity Fc receptor for IgG1. J. Immunol. 144: 371-378, 1990. PubMed: 2136886 |
| Cellular Products： | interleukin-1 (interleukin 1, IL-1, LAF); lysozyme |
| Comments： | J774A.1 cells are active in antibody dependent phagocytosis Pubmed: 1101071. Their growth is inhibited by dextran sulfate, PPD and LPS Pubmed: 318922. They synthesize large amounts of lysozyme and exhibits minor cytolysis but predominantly antibody-dependent phagocytosis. Interleukin 1 beta (Il1b) is synthesized continuously by this line. Total RNA from this line is available as TS211734R (100µg)
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Subcultures are prepared by scraping.xa0For a 75 cm2 flask, remove all but 10 mL of the culture medium. Adjust volume accordingly for different culture vessels. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.xa0Dislodge cells from the flask substrate with a cell scraper, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Replace or add medium two or three times weekly |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
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| Population Doubling Time： | about 17 hours |
| Name of Depositor： | P Ralph |
| Deposited As： | Mus musculus |
| Year of Origin： | 1968 |
| References： | Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890 Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031 Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922 Sears DW, et al. Molecular cloning and expression of the mouse high affinity Fc receptor for IgG1. J. Immunol. 144: 371-378, 1990. PubMed: 2136886 Ralph P, et al. Reticulum cell sarcoma: an effector cell in antibody-dependent cell- mediated immunity. J. Immunol. 114: 898-905, 1975. PubMed: 1089721 Ralph P, Nakoinz I. Phagocytosis and cytolysis by a macrophage tumour and its cloned cell line. Nature 257: 393-394, 1975. PubMed: 1101071 Knowlton KU, et al. A mutation in the puff region of VP2 attenuates the myocarditic phenotype of an infectious cDNA of the woodruff variant of coxsackievirus B3. J. Virol. 70: 7811-7818, 1996. PubMed: 8892902 Schissel SL, et al. Zn2+-stimulated sphingomyelinase is secreted by many cell types and is a product of the acid sphingomyelinase gene. J. Biol. Chem. 271: 18431-18436, 1996. PubMed: 8702487 Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03. |
| Cross References： | Nucleotide (GenBank) : X84789 M.musculus mRNA for p38-2G4. |