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| 产品名称： | JC |
|---|---|
| 商品货号： | TS211736 |
| Organism： | Mus musculus, mouse |
| Tissue： | mammary gland |
| Cell Type： | Epithelial-like |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 1.5 years |
| Gender： | Female |
| Strain： | BALB/cRos |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 40; range = 26 to 100 |
| Derivation： | JC is an epithelial-like cell line established in 1983 from a spontaneous primary mammary adenocarcinoma along the milk line.
|
| Clinical Data： | female The original tumor was passaged once in female BALB/c mice, and the resulting tumors were harvested and passaged in vitro for six months. |
| Tumorigenic： | Yes |
| Effects： | Yes, produces tumors with papillary adenocarcinoma morphology in BALB/c mice |
| Comments： | Electron microscopic examination detected the presence of cellular tonofilaments and desmosomes.
No type B or C virus particles were detected, although intracisternal A particles were observed occasionally. The original tumor was passaged once in female BALB/c mice, and the resulting tumors were harvested and passaged in vitro for six months. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution and remove trypsin. Add fresh trypsin solution and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Population Doubling Time： | 12 to 16 hrs |
| Name of Depositor： | TM Chu |
| Deposited As： | Mus musculus |
| Year of Origin： | 1983 |
| References： | Capone PM, et al. Immunotherapy in a spontaneously developed murine mammary carcinoma with syngeneic monoclonal antibody. Cancer Immunol. Immunother. 25: 93-99, 1987. PubMed: 3664534 Chao TY, Chu TM. Characterization of a new spontaneously developed murine mammary adenocarcinoma in syngeneic BALB/c hosts. In Vitro Cell. Dev. Biol. 25: 621-626, 1989. PubMed: 2666384 |