微信小陈
微信小章| 产品名称： | J1-31 |
|---|---|
| 商品货号： | TS211738 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody is useful for immunofluorescence, immunocytochemistry, and Western blotting procedures. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Clinical Data： | Animals were immunized with human cerebral white matter from a multiple sclerosis (MS) patient. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human astrocyte protein |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human astrocyte protein |
| Comments： | Animals were immunized with human cerebral white matter from a multiple sclerosis (MS) patient. Spleen cells were fused with NS-1 myeloma cells. The antibody recognizes an intracellular protein antigen (MW 30000) expressed by human and rat astrocytes and other specialized glia. (Muller cells of the retina, Bergmann fibers of the cerebellar cortex, tanycytes of the hypothalamus and ciliated ependymal cells) in the central nervous system (CNS). This marker is distinct from glial fibrillary acidic protein (GFAP) and vimentin. Astrocytes in the vicinity of a CNS lesion show enhanced staining for J1-31 antigen. J1-31 monoclonal antibody recognizes a protein whose expression is increased following CNS injury. The antibody is useful for immunofluorescence, immunocytochemistry, and Western blotting procedures. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at a cell concentration between 0.5 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | mouse IgM |
| Name of Depositor： | SK Malhotra |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Singh R, et al. A new "marker" protein for astrocytes. Biosci. Rep. 6: 73-80, 1986. PubMed: 2421799 . . Neurosci. Res. Commun. 4: 25031, 1989. . . Dendron 1: 91-108, 1992. Predy R, et al. Enhanced expression of a protein antigen (J1-31 antigen, 30 kilodaltons) by reactive astrocytes in lacerated spinal cord. J. Neurosci. Res. 19: 397-404, 1988. PubMed: 3385801 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |