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微信小章| 产品名称： | J.RT3-T3.5 |
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| 商品货号： | TS211742 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | acute T cell leukemia |
| Gender： | male |
| Applications： | transfection host |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The J.RT3-T3.5 cell line is a derivative mutant of the Jurkat leukemia cell line.
The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM.
The line was produced by treatment with ethylmethanesulfonate and negative selection with OKT3 monoclonal antibody.
This is a mutant line derived from the E6-1 clone of Jurkat (ATCC TIB 152) that lacks the beta chain of the T cell antigen receptor. |
| Clinical Data： | male The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al. |
| Comments： | xa0J.RT3-T3.5 cells lack the beta chain of the T cell antigen receptor. The cells do not express either CD3 or the T cell receptor alpha/beta heterodimer on the surface. The cells may be useful for transfection studies involving the T cell receptor beta chain gene. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Medium Renewal: 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1xa0x 106 cells/mL. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | A Weiss |
| Deposited As： | Homo sapiens |
| References： | Ohashi PS, et al. Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer. Nature 316: 606-609, 1985. PubMed: 4033759 Weiss A, Stobo JD. Requirement for the coexpression of T3 and the T cell antigen receptor on a malignant human T cell line. J. Exp. Med. 160: 1284-1299, 1984. PubMed: 6208306 Schneider U, et al. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. Int. J. Cancer 19: 621-626, 1977. PubMed: 68013 Ronald Wange, personal communication |