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| 产品名称： | J1j.10 |
|---|---|
| 商品货号： | TS211743 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against Thy-1.2 antigen; cytotoxic |
| Cellular Products： | immunoglobulin; monoclonal antibody; against Thy-1.2 antigen; cytotoxic |
| Tumorigenic： | Yes |
| Effects： | Yes, in immunosuppressed (C57BL/B6 X CBA)F1 mice |
| Comments： | Animals were immunized with C3H/HeJ thymocytes. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody does not lyse Thy-1.1 cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Modified Dulbeccos medium, 90%; horse serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype： | IgM |
| Name of Depositor： | J Sprent |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Bruce J, et al. A monoclonal antibody discriminating between subsets of T and B cells. J. Immunol. 127: 2496-2501, 1981. PubMed: 6975326 |