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微信小章| 产品名称： | J45.01 |
|---|---|
| 商品货号： | TS211744 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | acute T cell leukemia |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | CD45 deficient cells were isolated using a fluorescence activated cell sorter, and cloned by limiting dilution.
The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM.
The line was isolated after gamma irradiation of E6-1 followed by lysis of CD45 positive cells with the GAP 8.3 monoclonal antibody (see ATCC HB-12) and complement. |
| Clinical Data： | The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al. male |
| Antigen Expression： | CD2 +; CD3 +; CD28 +; CD45 - |
| Receptor Expression： | T cell antigen receptor
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| Genes Expressed： | produce interleukin 2 (IL-2) in response to calcium ionophores and phorbol esters |
| Cellular Products： | produce interleukin 2 (IL-2) in response to calcium ionophores and phorbol esters |
| Comments： | The J45.01 cell line is a CD45 deficient variant of the E6-1 clone of Jurkat (ATCC TIB-152).
CD45 deficient cells were isolated using a fluorescence activated cell sorter, and cloned by limiting dilution.
The cells express the T cell antigen receptor (TCR, CD3), CD2 and CD28 at levels comparable to the parental line, but only 5 to 8% as much CD45.
Loss of CD45 diminishes TCR - CD2 signal transduction, thus the cells are inducible for interleukin 2 (IL-2) production with calcium ionophores and phorbol esters, but not with TCr or CD2 stimulation. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mLand maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | GA Koretsky |
| Deposited As： | Homo sapiens |
| References： | Koretzky GA, et al. Tyrosine phosphatase CD45 is required for T-cell antigen receptor and CD2-mediated activation of a protein tyrosine kinase and interleukin 2 production. Proc. Natl. Acad. Sci. USA 88: 2037-2041, 1991. PubMed: 1672451 Schneider U, et al. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. Int. J. Cancer 19: 621-626, 1977. PubMed: 68013 Ronald Wange, personal communication |