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微信小章| 产品名称： | J558 |
|---|---|
| 商品货号： | TS211745 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | plasmacytoma; myeloma |
| Strain： | BALB/c |
| Applications： | Tested and found negative for ectromelia virus (mousepox). The cells are sensitive to dexamethasone and cortisol. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | diploid |
| Derivation： | The plasmacytoma was induced with mineral oil in a BALB/c mouse. |
| Antigen Expression： | Antigen expression: H-2d; PC.1 |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against alpha-1,3-dextran,H-2d; PC.1 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against alpha-1,3-dextran |
| Comments： | The cells are sensitive to dexamethasone and cortisol.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal:xa0Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Isotype： | IgA; lambda light chain |
| Name of Depositor： | M Cohn |
| Deposited As： | Mus musculus |
| References： | Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304 Gehring U, et al. Glucocorticoid action on hybrid clones derived from cultured myeloma and lymphoma cell lines. Proc. Natl. Acad. Sci. USA 69: 3124-3127, 1972. PubMed: 4343956 Halpern MS, Coffman RL. Polymer formation and J chain synthesis in mouse plasmacytomas. J. Immunol. 109: 674-680, 1972. PubMed: 5074326 Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881 Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714 Carson D, Weigert M. Immunochemical analysis of the cross-reacting idiotypes of mouse myeloma proteins with anti-dextran activity and normal anti-dextran antibody. Proc. Natl. Acad. Sci. USA 70: 235-239, 1973. PubMed: 4119224 Lundblad A, et al. Immunochemical studies on mouse myeloma proteins with specificity for dextran or for levan. Immunochemistry 9: 535-544, 1972. PubMed: 5036150 Cesari IM, Weigert M. Mouse lambda-chain sequences. Proc. Natl. Acad. Sci. USA 70: 2112-2116, 1973. PubMed: 4516208 Weigert MG, et al. Variability in the lambda light chain sequences of mouse antibody. Nature 228: 1045-1047, 1970. PubMed: 5483159 |