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微信小章| 产品名称： | intestine 407 |
|---|---|
| 商品货号： | TS211748 |
| Organism： | Homo sapiens, human |
| Tissue： | HeLa contaminant |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain human papilloma virus (HPV-18)
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination. HeLa Marker Chromosomes: One copy of M1, one copy of M2, four copies of M3 and one copy of M4 as revealed by G-banding patterns. |
| Derivation： | This line was originally thought to be derived from normal embryonic intestinal tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. |
| Clinical Data： | female |
| HeLa Markers： | Y |
| Genes Expressed： | keratin |
| Cellular Products： | keratin |
| Tumorigenic： | Yes |
| Effects： | Yes, in hamster (untreated) cheek pouch Yes, in immunosuppressed rats |
| Virus Susceptibility： | Human poliovirus 1
Human poliovirus 2 Human poliovirus 3 Vesicular stomatitis, Orsay (Indiana) Vesicular stomatitis, Glasgow (Indiana) Human adenovirus 3 Herpes simplex virus Pseudorabies virus Vaccinia virus Human coxsackievirus B1 Human Coxsackievirus B 2 Human Coxsackievirus B3 Human Coxsackievirus B4 Human Coxsackievirus B 5 Human Coxsackievirus B 6 Mumps rubulavirus , Mumps virus Newcastle disease virus |
| Virus Resistance： | rabiesvirus |
| Comments： | The cells are positive for keratin by immunoperoxidase staining |
| Complete Growth Medium： | Basal medium (Eagle) in Earles BSS, 90%; fetal bovine serum, 10%
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 9,10 D13S317: 12,13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
| Isoenzymes： | G6PD, A |
| Name of Depositor： | G Henle, F Deinhardt |
| Deposited As： | Homo sapiens |
| Year of Origin： | January, 1955 |
| References： | Henle G, Deinhardt F. The establishment of strains of human cells in tissue culture. J. Immunol. 79: 54-59, 1957. PubMed: 13475808 Lavappa KS. Survey of ATCC stocks of human cell lines for HeLa contamination. In Vitro 14: 469-475, 1978. PubMed: 566722 Lavappa KS, et al. Examination of ATCC stocks for HeLa marker chromosomes in human cell lines. Nature 259: 211-213, 1976. PubMed: 1250349 St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830 White LJ, et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293 |