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微信小章| 产品名称： | Indian Muntjac |
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| 商品货号： | TS211749 |
| Organism： | muntjac |
| Tissue： | skin |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain Bovine Viral Diarrhea Virus (BVDV)
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | adult |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 7; range = 7 to 14. Stable karyotype in male diploid stemline number. Chromosome breaks noted in 6% of the cells examined. |
| Virus Susceptibility： | Herpes simplex virus
Vaccinia virus Vesicular stomatitis virus Human poliovirus 1 |
| Comments： | Recent tests for bovine viral diarrhea virus (BVDV) indicate that the line is producing both detectable BVDV antigens and infectious BVDV virions (J. Virol Methods 48:211-221, 1994). |
| Complete Growth Medium： | Hams F10 medium, 80%; fetal bovine serum, 20%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:4 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
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| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | DH Wunsten |
| Deposited As： | muntjac |
| Year of Origin： | November, 1969 |
| References： | Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438 Wurster DH, Benirschke K. Indian muntjac, Muntiacus muntjak: a deer with a low diploid chromosome number. Science 168: 1364-1366, 1970. PubMed: 5444269 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |