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| 产品名称： | IVD12 |
|---|---|
| 商品货号： | TS211750 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody identifies the HLA DQw3 supertypic specificity. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against HLA DQ |
| Cellular Products： | immunoglobulin; monoclonal antibody; against HLA DQ |
| Comments： | Animals were immunized with the PRIESS human B cell line. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody identifies the HLA DQw3 supertypic specificity. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Culture Medium: Dulbeccos modified Eagles medium with 4.5 g/L glucose and 1 mM sodium pyruvate, 85%; fetal bovine serum, 15%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | JD Capra, RC Giles, CK Hurley |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Giles RC, et al. Structural analysis of a human I-A homologue using a monoclonal antibody that recognizes an MB3-like specificity. J. Exp. Med. 157: 1461-1470, 1983. PubMed: 6189938 |