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微信小章| 产品名称： | IP2-E4 |
|---|---|
| 商品货号： | TS211751 |
| Organism： | Mus musculus, mouse |
| Tissue： | axillary lymph node; vascular epithelium |
| Cell Type： | endothelial, SV40 transformed |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Gender： | male |
| Strain： | C3H/HeJ |
| Applications： | The IP2-E4 cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161). IP2-E4 cells are sensitive to lysis by activated macrophage as measured in the chromium release assay. They do not express high levels of vascular cell adhesion molecule (VCAM) constitutively however, stimulation with TNF alpha caused an up regulation of VCAM expression. This clone retains the ability to differentiate on a synthetic basement-like membrane. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The IP2-E4 cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161). |
| Clinical Data： | male |
| Antigen Expression： | H-2 K; VCAM |
| Genes Expressed： | H-2 K; VCAM |
| Tumorigenic： | Yes |
| Effects： | Yes, the cells induced spindle tumors in nude mice with some of the histopathologic characteristics of human Kaposi Sarcoma after a shortened latency period of approximately 2 weeks |
| Comments： | The IP2-E4 cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161). The line was cloned in 1992 by limiting dilution. IP2-E4 cells are sensitive to lysis by activated macrophage as measured in the chromium release assay. The cells express the cell surface major histocompatibility complex class I antigen, H-2 k of the parental cell line. They do not express high levels of vascular cell adhesion molecule (VCAM) constitutively however, stimulation with TNF alpha caused an up regulation of VCAM expression. This clone retains the ability to differentiate on a synthetic basement-like membrane. The cells stain positively for SV40 T antigen. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:6 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by Wiley-Freshney, 5th edition, published by Alan R. Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | KA OConnell |
| References： | OConnell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170 OConnell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposis sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612 OConnell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposis sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |