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| 产品名称： | J.gamma1.WT |
|---|---|
| 商品货号： | TS211756 |
| Organism： | Homo sapiens, human |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | clusters in suspension |
| Biosafety Level： | 2 xa0Cells contain CMV and SV-40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | acute T cell leukemia |
| Gender： | male |
| Applications： | The J.gamma1.WT cell line was derived from the phospholipase C-gamma1 (PLC-gamma1) deficient J.gamma1 cell line (ATCC CRL-2678) by stable transfection with a wild-type expression vector, pcaPLC-gamma1. The J.gamma1.WT derivative (TS211756) stably expresses PLC-gamma1at levels comparable to the Jurkat cell line. |
| Derivation： | The J.gamma1.WT cell line was derived from the phospholipase C-gamma1 (PLC-gamma1) deficient J.gamma1 cell line (ATCC CRL-2678) by stable transfection with a wild-type expression vector, pcaPLC-gamma1. The J.gamma1.WT derivative (TS211756) stably expresses PLC-gamma1at levels comparable to the Jurkat cell line. |
| Clinical Data： | male |
| Comments： | The J.gamma1.WT cell line was derived from the phospholipase C-gamma1 (PLC-gamma1) deficient J.gamma1 cell line (ATCC CRL-2678) by stable transfection with a wild-type expression vector, pcaPLC-gamma1. The vector contains cytomegalovirus (CMV) and SV40 viral DNA sequences and the neomycin resistance gene. The J.gamma1.WT derivative (TS211756) stably expresses PLC-gamma1at levels comparable to the Jurkat cell line. |
| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.4 mg/ml G418, 90%; fetal bovine serum, 10%
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| Subculturing： | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml. Maintain cell density between 3 X 10 exp5 and 2 X 10 exp6 viable cells/ml. |
| Cryopreservation： | culture medium, 45%; fetal bovine serum, 45%; DMSO, 10% |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | RT Abraham |
| Deposited As： | human |
| References： | Irvin BJ, et al. Pleiotropic contributions of phospholipase C-gamma1 (PLC-gamma1) to T-cell antigen receptor-mediated signaling reconstitution studies of a PLC-gamma1-deficient Jurkat T-cell line. Mol. Cell. Biol. 20: 9149-9161, 2000. PubMed: 11094067 |