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微信小章| 产品名称： | J.CaM1.6 (derivative mutant of Jurkat) |
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| 商品货号： | TS211757 |
| Organism： | Homo sapiens, human |
| Tissue： | Peripheral blood |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | acute T cell leukemia |
| Gender： | male |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The J.CaM1.6 cell line is a derivative mutant of Jurkat. The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM. The J.CaM1.6 line was derived from the E6-1 clone of Jurkat (ATCC TIB-152) by treatment with ethylmethanesulfonate (EMS). Cells were then selected for T cell antigen receptor (TCR) expression and inability to increase intracellular calcium in response to TCR stimulation with OKT3 antibody. |
| Clinical Data： | male
14 years |
| Comments： | CD3/T1 complexes are present; however, TCR signal transduction is defective. They are deficient in lck kinase activity and are missing exon 7 in their lck mRNA. The T cell antigen receptor appears to be structurally normal. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 105 viable cells/mL. Maintain cell density between 2 X 105 and 2 X 106 viable cells/mL.
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11 D13S317: 8,12 D16S539: 10,11 D5S818: 9 D7S820: 8,10 THO1: 6,9.3 TPOX: 8,10 vWA: 18,19 |
| Name of Depositor： | A Weiss |
| Deposited As： | Homo sapiens |
| References： | Straus DB, Weiss A. Genetic evidence for the involvement of the lck tyrosine kinase in signal transduction through the T cell antigen receptor. Cell 70: 585-593, 1992. PubMed: 1505025 Goldsmith MA, Weiss A. Isolation and characterization of a T-lymphocyte somatic mutant with altered signal transduction by the antigen receptor. Proc. Natl. Acad. Sci. USA 84: 6879-6883, 1987. PubMed: 3309950 Lund T, et al. Herpesvirus saimiri Tip-484 membrane protein markedly increases p56lck activity in T cells. J. Virol. 71: 378-382, 1997. PubMed: 8985360 Cenciarelli C, et al. T cell antigen receptor ubiquitination is a consequence of receptor-mediated tyrosine kinase activation. J. Biol. Chem. 271: 8709-8713, 1996. PubMed: 8621503 Gibson S, et al. Functional LCK is required for optimal CD28-mediated activation of the TEC family tyrosine kinase EMT/ITK. J. Biol. Chem. 271: 7079-7083, 1996. PubMed: 8636141 Hutchcroft JE, et al. Differential phosphorylation of the T lymphocyte costimulatory receptor CD28. J. Biol. Chem. 271: 13362-13370, 1996. PubMed: 8662792 Schneider U, et al. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. Int. J. Cancer 19: 621-626, 1977. PubMed: 68013 Ronald Wange, personal communication |