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| 产品名称： | IL-A51 |
|---|---|
| 商品货号： | TS211759 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with a 34000 to 35000 dalton antigen on bovine T cells that is equivalent to human CD8. This is one of a series of hybridomas produced by Dr. |
| Derivation： | Spleen cells were fused with P3X63Ag8 myeloma cells. This is one of a series of hybridomas produced by Dr. W. Ivan Morrison at the International Laboratory for Research on Animal Diseases (ILRAD). |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against bovine CD8 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against bovine CD8 |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | Animals were immunized with bovine lymphocytes. Spleen cells were fused with P3X63Ag8 myeloma cells. The antibody reacts with a 34000 to 35000 dalton antigen on bovine T cells that is equivalent to human CD8. This is one of a series of hybridomas produced by Dr. W. Ivan Morrison at the International Laboratory for Research on Animal Diseases (ILRAD). |
| Complete Growth Medium： | RPMI 1640 medium with 0.02 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | FA Osorio, WI Morrison |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Clevers H, et al. Identification of a bovine surface antigen uniquely expressed on CD4- CD8- T cell receptor gamma/delta+ T lymphocytes. Eur. J. Immunol. 20: 809-817, 1990. PubMed: 1971793 Ellis JA, et al. Characterization by a monoclonal antibody and functional analysis of a subset of bovine T lymphocytes that express BoT8, a molecule analogous to human CD8. Immunology 58: 351-358, 1986. PubMed: 2426182 |