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微信小章| 产品名称： | IM-9 |
|---|---|
| 商品货号： | TS211766 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | B lymphoblast; Epstein-Barr virus (EBV) transformed |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells contain Herpesvirus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | multiple myeloma |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | normal human female; diploid; stable; Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Derivation： | Although derived from the blood of a patient with multiple myeloma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line. |
| Clinical Data： | Caucasian
female |
| Antigen Expression： | CD11a +; CD19 +; CD20 +; CD38 -; CD49e + |
| Receptor Expression： | growth hormone receptor
insulin receptor
calcitonin receptor
|
| Genes Expressed： | immunoglobulin |
| Cellular Products： | immunoglobulin |
| Comments： | The cells are EBNA positive.xa0 ATCC confirmed this cell line is positive for the presence of Epstein-Barr (EBV) viral DNA sequences via PCR. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1xa0to 2xa0x 105 viable cells/mL. Maintain cell density between 1x 105 and 1xa0x 106 viable cells/mL. Do not allow the cell density to exceed 2xa0x 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,11 D13S317: 9,11 D16S539: 9,13 D5S818: 13 D7S820: 11,12 THO1: 6,9.3 TPOX: 11 vWA: 14,17 |
| Isoenzymes： | ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 2 PGM1, 1-2 PGM3, 0 |
| Isotype： | IgG kappa , IgG; kappa light chain
Ref  van Boxel JA, Buell DN. IgD on cell membranes of human lymphoid cell lines with multiple immunoglobulin classes. Nature 251: 443-444, 1974. PubMed: 4608784 |
| Name of Depositor： | DN Buell |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1967 |
| References： | Fahey JL, et al. Proliferation and differentiation of lymphoid cells: studies with human lymphoid cell lines and immunoglobulin synthesis. Ann. N.Y. Acad. Sci. 190: 221-234, 1971. PubMed: 5290016 Lesniak MA, et al. Binding of 125I-human growth hormone to specific receptors in human cultured lymphocytes. Characterization of the interaction and a sensitive radioreceptor assay. J. Biol. Chem. 249: 1661-1667, 1974. PubMed: 4361817 Van Obberghen E, et al. Cell surface receptors for insulin and human growth hormone. Effect of microtubule and microfilament modifiers. J. Biol. Chem. 251: 6844-6851, 1976. PubMed: 185222 Gavin JR, et al. Insulin-dependent regulation of insulin receptor concentrations: a direct demonstration in cell culture. Proc. Natl. Acad. Sci. USA 71: 84-88, 1974. PubMed: 4359334 van Boxel JA, Buell DN. IgD on cell membranes of human lymphoid cell lines with multiple immunoglobulin classes. Nature 251: 443-444, 1974. PubMed: 4608784 Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375 Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762 van Boxel JA, Buell DN. IgD on cell membranes of human lymphoid cell lines with multiple immunoglobulin classes. Nature 251: 443-444, 1974. PubMed: 4608784 |
| Cross References： | Nucleotide (GenBank) : X81121 H.sapiens mRNA for central cannabinoid receptor, short isoform. Nucleotide (GenBank) : X84369 H.sapiens RNA for heavy chain variable and constant regions (IM9-VH). Nucleotide (GenBank) : U07989 Human Burkitts lymphoma immunoglobulin kappa light chain mRNA, partial cds. Nucleotide (GenBank) : U07985 Human myeloma immunoglobulin heavy chain Fd region V-D-J-CH1 mRNA, partial cds. |