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微信小章| 产品名称： | IM7.8.1 |
|---|---|
| 商品货号： | TS211767 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody may be useful for detecting subclasses of intrathymic progenitor cells and in detecting memory T cells in the periphery. |
| Derivation： | Spleen cells were fused with S194/5.XXO.BU.1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a non-polymorphic determinant on mouse pgp-1 glycoprotein |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a non-polymorphic determinant on mouse pgp-1 glycoprotein |
| Comments： | Animals were immunized with dexamethasone induced cells of the M1 mouse myeloid cell line. Spleen cells were fused with S194/5.XXO.BU.1 myeloma cells. The antibody may be useful for detecting subclasses of intrathymic progenitor cells and in detecting memory T cells in the periphery. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium, 90%; heat-inactivated horse serum, 10%
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2b |
| Name of Depositor： | R Hyman |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Budd RC, et al. Distinction of virgin and memory T lymphocytes. Stable acquisition of the Pgp-1 glycoprotein concomitant with antigenic stimulation. J. Immunol. 138: 3120-3129, 1987. PubMed: 3106474 Trowbridge IS, et al. Biochemical characterization and cellular distribution of a polymorphic, murine cell-surface glycoprotein expressed on lymphoid tissues. Immunogenetics 15: 299-312, 1982. PubMed: 7068174 Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913 Lesley J, et al. Qualitative and quantitative heterogeneity in Pgp-1 expression among murine thymocytes. Cell. Immunol. 112: 40-54, 1988. PubMed: 2894254 Lesley J, et al. Kinetics of thymus repopulation by intrathymic progenitors after intravenous injection: Evidence for successive repopulation by an IL-2R+, Pgp-1- and by an IL-2R-, Pgp-1+ progenitor. Cell. Immunol. 117: 378-388, 1988. PubMed: 3058318 |