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微信小章| 产品名称： | IgG-1D2 |
|---|---|
| 商品货号： | TS211769 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized against the GST-human SREBP-2 fusion protein (amino acids 48 to 403). The antibody recognizes both the precursor and mature forms of SREBP-2 in cultured human cells, and can be used in immunoblotting and ELISA assays to study SREBP-2 in cultured human cells grown under different conditions. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human sterol regulatory element binding protein 2 (SREBP 2, SREBP-2) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human sterol regulatory element binding protein 2 (SREBP 2, SREBP-2) |
| Comments： | Animals were immunized against the GST-human SREBP-2 fusion protein (amino acids 48 to 403). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody recognizes both the precursor and mature forms of SREBP-2 in cultured human cells, and can be used in immunoblotting and ELISA assays to study SREBP-2 in cultured human cells grown under different conditions. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. Medium Renewal:xa0 Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | JL Goldstein, YK Ho |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Janowski BA, et al. The hypocholesterolemic agent LY295427 reverses suppression of sterol regulatory element-binding protein processing mediated by oxysterols. J. Biol. Chem. 48: 45408-45416, 2001. PubMed: 11577112 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |