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| 产品名称： | IgG-1C6 |
|---|---|
| 商品货号： | TS211771 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized with a bacterially produced fusion protein encoding 6 consecutive histidines followed by amino acids 833 to 1141 of human SREBP. The antibody recognizes the carboxy terminal portion of human SREBP-2, and can be used in immunoblotting assays to detect carboxy terminal fragments of SREBP-2. |
| Derivation： | Animals were immunized with a bacterially produced fusion protein encoding 6 consecutive histidines followed by amino acids 833 to 1141 of human SREBP. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human sterol regulatory element binding protein 2 (SREBP 2, SREBP-2) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human sterol regulatory element binding protein 2 (SREBP 2, SREBP-2) |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | Animals were immunized with a bacterially produced fusion protein encoding 6 consecutive histidines followed by amino acids 833 to 1141 of human SREBP. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody recognizes the carboxy terminal portion of human SREBP-2, and can be used in immunoblotting assays to detect carboxy terminal fragments of SREBP-2. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1 |
| Name of Depositor： | JL Goldstein, YK Ho |
| Deposited As： | mouse (B cell); mouse (myeloma) |