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微信小章| 产品名称： | IgG-9D5 |
|---|---|
| 商品货号： | TS211776 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The hybridoma cell line IgG-9D5 secretes a mouse monoclonal antibody (IgG2b) that recognizes hamster SCAP (SREBP sterol regulatory element binding protein cleavage-activating protein. The antibody is useful in immunoblot and immunoprecipitation of SCAP in cell extracts from cells grown in different conditions; the results help to understand the domain interactions between SREBP and SCAP. The line was produced by fusing Sp2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized against a fusion protein encoding 6 consecutive histidines followed by amino acids 540-707 of hamster SCAP. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was produced by fusing Sp2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized against a fusion protein encoding 6 consecutive histidines followed by amino acids 540-707 of hamster SCAP. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against SCAP (SREBP sterol regulatory element binding protein cleavage activating protein) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against SCAP (SREBP sterol regulatory element binding protein cleavage activating protein) |
| Comments： | The hybridoma cell line IgG-9D5 secretes a mouse monoclonal antibody (IgG2b) that recognizes hamster SCAP (SREBP sterol regulatory element binding protein cleavage-activating protein.) SCAP regulates cholesterol metabolism by stimulating cleavage of the transcription factors SREBP-1 and SREBP-2 in cultured mammalian cells. The antibody is useful in immunoblot and immunoprecipitation of SCAP in cell extracts from cells grown in different conditions; the results help to understand the domain interactions between SREBP and SCAP. The line was produced by fusing Sp2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized against a fusion protein encoding 6 consecutive histidines followed by amino acids 540-707 of hamster SCAP. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2xa0 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days depending on cell density. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG2b |
| Name of Depositor： | JL Goldstein, YK Ho |
| References： | Hua X, et al. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein. Cell 87: 415-426, 1996. PubMed: 8898195 Sakai J, et al. Identification of complexes between the COOH-terminal domains of sterol regulatory element-binding proteins (SHEBPs) and SREBP cleavage-activating protein. J. Biol. Chem. 272: 20213-20221, 1997. PubMed: 9242699 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |