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微信小章| 产品名称： | IgH-2 |
|---|---|
| 商品货号： | TS211777 |
| Organism： | iguana |
| Tissue： | heart |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | immature |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 35; range = 30 to 64. The karyotype is stable. There is one large marker chromosome with a terminal centromere appearing in all cells in addition to 22 minute chromosomes, 10 large chromosomes with a median centromere and 2 large chromosomes with a submedian centromere. |
| Virus Susceptibility： | iguana
Herpes simplex virus Pseudorabies virus Vaccinia virus Human poliovirus 1 Vesicular stomatitis virus |
| Complete Growth Medium： | Eagless Minimum Essential Medium with Hanks BSS, 2 mM L-glutamine, and 0.1 mM non-essential amino acids supplemented with 10% fetal bovine serum
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Complete growth medium 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
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| Culture Conditions： | Temperature: 36°C
Atmosphere: Air, 100%; no CO2 |
| Name of Depositor： | HF Clark |
| Deposited As： | iguana |
| Year of Origin： | August, 1966 |
| References： | Cohen MM, et al. The somatic chromosomes of 3 lizard species: Gekko gecko, Iguana iguana, and Crotaphytus collaris. Experientia 23: 769-771, 1967. PubMed: 6062904 Clark HF, et al. Characterization of reptilian cell lines established at incubation temperatures of 23 to 36 degrees. Proc. Soc. Exp. Biol. Med. 133: 1039-1047, 1970. PubMed: 4314147 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
| Cross References： | Nucleotide (GenBank) : L27530 Iguana small cytoplasmic RNA (Y3). Nucleotide (GenBank) : L27537 Iguana small cytoplasmic RNA (Y4). Nucleotide (GenBank) : L27532 Iguana small cytoplasmic RNA (Y3) gene. Nucleotide (GenBank) : L27534 Iguana small cytoplasmic RNA (Y4) gene. Nucleotide (GenBank) : L27536 Iguana small cytoplasmic RNA (Y4) gene. |